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Molecular Cell Biology Research Group (supported by the Momentum Program of HAS)

Group Leader:

László Homolya, D.Sc.            Senior Scientist

Lab Members:

Ágota Apáti, Ph.D.                  Senior Research Associate

Zsuzsa Bartos                         Ph.D. student

Gyöngyi Bézsenyi                   Technician

Beáta Haraszti                        Technician

Zoltán Hegyi                           Junior Research Associate

Éva Kosóczki                           Junior Research Associate

Barbara Orsolits, Ph.D.          Postdoctoral Fellow

Dóra Reé                                Biotechnologist

Katalin Rieth                           Technician

György Török                          Junior Research Associate

György Várady, Ph.D.             Senior Research Associate

Nóra Varga                             Junior Research Associate

Anikó Zeöld, Ph.D.                  Postdoctoral Fellow

Undergraduates, volunteers:

Berecz Tünde                         Student

Lilienberg Julianna                 Student

Szebényi Kornélia, Ph.D.        Volunteer

Research:

The Molecular Cell Biology Research Group has been established in 2012 with the support of the Momentum Program of the Hungarian Academy of Sciences. The research topics of the group comprise the biochemical and cell biological investigation of membrane transporters (primarily ABC transporters) in polarized cells; the cell biological characterization of human pluripotent stem cells and their progenies, as well as their exploitation in various model systems; and the utilization of membrane transporters as diagnostic tools. In accordance with these three different but interrelated research focuses, the Research Group is subdivided into three subgroups: the Laboratory of Trafficking (headed by László Homolya), the Pluripotent Stem Cell Laboratory (headed by Ágota Apáti), and the Flow Cytometry Laboratory (headed by György Várady). We use molecular biological, biochemical and cell biological approaches to investigate the transporters, the regulation of their localization and their function, as well as the Ca2+ signaling in various cellular model systems, such as different cancer cell lines, primary cultures of isolated mouse and rat cells, human pluripotent stem cell lines, and their differentiated daughter cells. For the development of diagnostic methodologies, isolated blood cells, primarily red blood cells are used. In our basic research we always intend to investigate such problems, which have biomedical relevance and can fuel further developments for diagnostic or therapeutic use.

Publications (2012-2016)

Journal papers:

  1. Hegyi, Z., Homolya, L.: Functional Cooperativity between ABCG4 and ABCG1 Isoforms.PLoS One 11(5): e0156516 (2016)                                                                                                                                                                                                                                                                IF: 3,234
  2. Telbisz, Á., Homolya, L. Recent advances in the exploration of the bile salt export pump (BSEP/ABCB11) function. Expert Opin Ther Targets 20(4):501-14.  (2016)          IF: 5.139
  3. Péntek A, Pászty K, Apáti Á.: Analysis of Intracellular Calcium Signaling in Human Embryonic Stem Cells. Methods Mol Biol. 1307:141-7. (2016)                                           IF: 1,290
  4. Kecse-Nagy, C., Szittner, Z., Papp†, k., Hegyi, Z., Paolo Rovero, P., Migliorini, P., Lóránd, V, Homolya, L., Prechl, J.: Characterization of NF-κB reporter U937 cells and their application for the detection of inflammatory immune-complexes. PLoS One 11(5): e0156328 (2016)                                                                                                                                  IF: 3,234
  5. Bátai-Konczos, A., Veres, Z., Szabó, M., Ioja, E., László, G., Török, G., Homolya, L., Jemnitz, K.: Comparative study of CYP2B1/2 induction and the transport of bilirubin and taurocholate in rat hepatocyte-mono- and hepatocyte-Kupffer cell co-cultures. J Pharmacol Toxicol Methods. pii: S1056-8719(16)30055-7. doi: 10.1016/j.vascn. (2016)                                                                                                                                                                                                                                                                                                                                                       IF: 2,390
  6. Nyeste, A., Bencsura, P., Vida I., Hegyi, Z., Homolya, L., Fodor, E., Welker E.: Expression of the prion protein family member Shadoo causes drug hypersensitivity that is diminished by the coexpression of the wild type prion protein. J Biol Chem. – 26;291(9):4473-86. (2016)             IF: 4.573
  7. Szebenyi, K., Füredi, A., Kolacsek, O., Pergel, E., Bosze, Z., Bender, B., Vajdovich, P., Tovari, J., Homolya, L., Szakacs, G., Heja, L., Enyedi, A., Sarkadi, B., Apati, A., Orban, T.I.: Generation of a Homozygous Transgenic Rat Strain Stably Expressing a Calcium Sensor Protein for Direct Examination of Calcium Signaling. Sci Rep. 5: 12645 (2015)                                                                          IF: 5.578
  8. Apáti Á, Szebényi K, Erdei Z, Várady G, Orbán TI, Sarkadi B: The importance of drug transporters in human pluripotent stem cells and in early tissue differentiation. Expert Opin Drug Metab Toxicol. 14:1-16. (2015)                                                                                                      IF: 2,831
  9. Szebényi K, Füredi A, Kolacsek O, Csohány R, Prókai Á, Kis-Petik K, Szabó A, Bősze Z, Bender B, Tóvári J, Enyedi Á, Orbán TI, Apáti Á, Sarkadi B: Visualization of calcium dynamics in rat kidney proximal tubules. J Am Soc Nephrol. 26(11):2731-40. (2015)                       IF: 9,340
  10. Erdélyi-Belle B, Török G, Apáti Á, Sarkadi B, Schaff Z, Kiss A, Homolya L.: Expression of Tight Junction Components in Hepatocyte-Like Cells Differentiated from Human Embryonic Stem Cells. Pathol Oncol Res. Sep;21(4):1059-70. (2015)                                   IF: 1,674
  11. Szebényi K, Péntek A, Erdei Z, Várady G, Orbán TI, Sarkadi B, Apáti A: Efficient Generation of Human Embryonic Stem Cell-Derived Cardiac Progenitors Based on Tissue-Specific EGFP Expression. Tissue Engineering Part C Methods. 21(1):35-45. (2015)                            IF: 4,448
  12. Ribiczey, P., Papp, B., Homolya, L., Enyedi, Á., Kovács, T. Selective upregulation of the expression of plasma membrane calcium ATPase isoforms upon differentiation and 1,25(OH)2D3-vitamin treatment of colon cancer cells. Biochem Biophys Res Commun. 464(1): 189-194 (2015)  IF: 2.297
  13. Ma X, Lubin H, Ioja E, Kékesi O, Simon Á, Apáti Á, Orbán TI, Héja L, Kardos J, Markó IE: Straightforward and effective synthesis of γ-aminobutyric acid transporter subtype 2-selective acyl-substituted azaspiro [4.5] decanes. Bioorg Medicinal Chem Let. 15;26(2):417-23. (2015) IF: 2,420
  14. Bacskai I, Mázló A, Kis-Tóth K, Szabó A, Panyi G, Sarkadi B, Apáti Á, Rajnavölgyi É.: Mesenchymal Stromal Cell-Like Cells Set the Balance of Stimulatory and Inhibitory Signals in Monocyte-Derived Dendritic Cells. Stem Cells Dev. 1;24(15):1805-16. (2015)                            IF: 4,055
  15. Várady G, Szabó E, Fehér Á, Németh A, Zámbó B, Pákáski M, Janka Z, Sarkadi B.: Alterations of membrane protein expression in red blood cells of Alzheimer’s disease patients. Alzheimers Dement (Amst) 1:(3) pp. 334-338. (2015)                                                                                                   IF: N/A
  16. Hegedus T, Chaubey PM, Varady G, Szabo E, Saranko H, Hofstetter L, Roschitzki B, Stieger B, Sarkadi B: Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford).  p. bav056. (2015)                        IF: 3,372
  17. Merkely B, Gara E, Lendvai Z, Skopal J, Leja T, Zhou W, Kosztin A, Varady G, Mioulane M, Bagyura Z, Nemeth T, Harding SE, Foldes G.: Signalling via PI3K/FOXO1A Pathway Modulates Formation and Survival of Human Embryonic Stem Cell-Derived Endothelial Cells. Stem Cells Dev 24:(7) pp. 869-878. (2015)                  IF: 3,727
  18. Szepesi A, Matula Z, Szigeti A, Varady G, Szabo G, Uher F, Sarkadi B, Nemet K.: ABCG2 Is a Selectable Marker for Enhanced Multilineage Differentiation Potential in Periodontal Ligament Stem Cells. Stem Cell Dev 24:(2) pp. 244-252. (2015)                                                        IF: 3,727
  19. Kolacsek O, Erdei Z, Apáti A, Sándor S, Izsvák Z, Ivics Z, Sarkadi B, Orbán TI: Excision efficiency is not strongly coupled to transgenic rate: cell type dependent transposition efficiency of Sleeping Beauty and piggyBac DNA transposons. Human Gene Therapy Methods. 25(4):241-52. (2014)               IF: 2,436
  20. Erdei Z, Lőrincz R, Szebényi K, Péntek A, Varga N, Likó I, Várady G, Szakács G, Orbán TI, Sarkadi B, Apáti A: Expression pattern of the human ABC transporters in pluripotent embryonic stem cells and in their derivatives. Cytometry Part B: Clinical Cytometry. 86(5):299-310. (2014)                              IF: 2,398
  21. Koszarska M, Kucsma N, Kiss K, Varady G, Gera M, Antalffy G, Andrikovics H, Tordai A, Studzian M, Strapagiel D, Pulaski L, Tani Y, Sarkadi B, Szakacs G.: Screening the Expression of ABCB6 in Erythrocytes Reveals an Unexpectedly High Frequency of Lan Mutations in Healthy Individuals. PLoS One 9(10): p. e111590. (2014)                                                                                                    IF: 3,234
  22. Homolya, L., Fu, D., Sengupta, P., Jarnik, M., Gillet, J.-P., Vitale-Cross, L., Gutkind, J.S., Lippincott-Schwartz, J., Arias, I.M. LKB1/AMPK and PKA Control ABCB11 trafficking and polarization in hepatocytes. PLoS ONE 9(3): e91921 (2014)                                                           IF: 3.534
  23. Kolacsek O, Krízsik V, Schamberger A, Erdei Z, Apáti A, Várady G, Mátés L, Izsvák Z, Ivics Z, Sarkadi B, Orbán TI.: Erratum: Reliable transgene-independent method for determining sleeping beauty transposon copy numbers. Mobile DNA 4 (11) Mobile DNA 4:(1) Paper 11. (2013)               IF: 2,429
  24. Várady György, Cserepes Judit, Németh Adrienn, Szabó Edit, Sarkadi Balázs.: Cell surface membrane proteins as personalized biomarkers: where we stand and where we are headed. Biomark Med. 7:(5) pp. 803-819. (2013)                                                                                            IF: 2,858
  25. Apáti Á, Pászty K, Hegedűs L, Kolacsek O, Orbán TI, Erdei Z, Szebényi K, Péntek A, Enyedi Á, Sarkadi B: Characterization of calcium signals in human embryonic stem cells and in their differentiated offspring by a stably integrated calcium indicator protein. Cellular Signalling. 25(4):752-9. (2013)                IF: 4,471
  26. Erdei Z, Sarkadi B, Brózik A, Szebényi K, Várady G, Makó V, Péntek A, Orbán TI, Apáti Á: Dynamic ABCG2 expression in human embryonic stem cells provides the basis for stress response. European Biophysics Journal. 42(2-3):169-79. (2013)                                                                             IF: 2,474
  27. Arbabian A, Brouland JP, Apáti Á, Pászty K, Hegedűs L, Enyedi Á, Chomienne C, Papp B.: Modulation of endoplasmic reticulum calcium pump expression during lung cancer cell differentiation. FEBS J. 280(21):5408-18. (2013)                                                                                                             IF: 3,850
  28. de Athayde Moncorvo Collado, A, Corbalán, N, Homolya, L, Morero, R, Minahk, C. Resveratrol modulates ATPase activity of liposome-reconstituted ABCG1.FEBS Lett. 587: 2359-63 (2013)                           IF: 3.582
  29. de Boussac H, Orban TI, Varady G, Tihanyi B, Bacquet C, Brozik A, Varadi A, Sarkadi B, Aranyi T.: Stimulus-induced expression of the ABCG2 multidrug transporter in HepG2 hepatocarcinoma model cells involves the ERK1/2 cascade and alternative promoters. Biochem Biophys Res Commun. 426:(2) pp. 172-176. (2012)                    IF: 2,406
  30. Hegedűs C, Truta-Feles K, Antalffy G, Várady G, Német K, Özvegy-Laczka C, Kéri G, Orfi L, Szakács G, Settleman J, Váradi A, Sarkadi B.: Interaction of the EGFR inhibitors gefitinib, vandetanib, pelitinib and neratinib with the ABCG2 multidrug transporter: Implications for the emergence and reversal of cancer drug resistance. Biochem Pharmacol. 84:(3) pp. 260-267. (2012)           IF: 4,576
  31. Kasza I, Varady G, Andrikovics H, Koszarska M, Tordai A, Scheffer GL, Nemeth A, Szakacs G, Sarkadi B.: Expression Levels of the ABCG2 Multidrug Transporter in Human Erythrocytes Correspond to Pharmacologically Relevant Genetic Variations. PLoS One 7(11): Paper e48423. 8 p. (2012)                     IF: 3,730
  32. Sarkadi B, Hegedus C, Truta-Feles K, Ozvegy-Laczka C, Telbisz A, Varady G. Interactions between the ABCG2 multidrug transporter and inhibitors of the EGFR signaling pathway. Faseb Journal 26:(799.6) Paper &. 1 p. (2012)                                                                                                                         IF: 5,704
  33. Telbisz A, Hegedus C, Ozvegy-Laczka C, Goda K, Varady G, Takats Z, Szabo E, Sorrentino BP, Varadi A, Sarkadi B. Antibody binding shift assay for rapid screening of drug interactions with the human ABCG2 multidrug transporter. Eur J Pharm Sci. 45:(1-2) pp. 101-109. (2012)        IF: 2,987
  34. Apáti Á, Pászty K, Erdei Z, Szebényi K, Homolya L, Sarkadi B.: Calcium signaling in pluripotent stem cells. Mol Cell Endocrinol. 28; 353(1-2):57-67. (2012)            IF: 3,794
  35. Papp B, Brouland JP, Arbabian A, Gélébart P, Kovács T, Bobe R, Enouf J, Varin-Blank N, Apáti A.: Endoplasmic reticulum calcium pumps and cancer cell differentiation. Biomolecules. 5;2(1):165-86. (2012)                          IF: 0,654
  36. Apáti, A, Pászty, K, Erdei, Z, Szebényi, K, Homolya, L, and Sarkadi, B. Calcium signaling in pluripotent stem cells. Mol Cell Endocrinol. 353(1-2): 57-67 (2012)  IF: 4.119

Book chapters:

  1. Váradi A., Homolya L. Two liver transporters, ABCB11 and ABCC6; novel therapeutic approaches in the related disorders In: ABC Transporters – 40 Years on. (ed. A. George) Springer International Publishing, pp. 1-18. ISBN: 9783319234755 (2016)
  2. Kornélia Szebényi, Adrienn Péntek, Nóra Varga, Zsuzsa Erdei, Gergő Vőfély, Tamás I. Orbán, Balázs Sarkadi, and Ágota Apáti: Human Stem-Cell-Derived Cardiomyocytes in Drug Discovery and Toxicity Testing. In: Handbook of Nanotoxicology, Nanomedicine and Stem Cell Use in Toxicology, Saura C. Sahu and Daniel A. Casciano (Eds.). John Wiley & Sons, Ltd, ISBN: 978-1-118-43926-5, 267-297. (2014)
  3. Tamás I. Orbán, Ágota Apáti, Zsuzsanna Izsvák, Zoltán Ivics and Balázs Sarkadi: Use of Transposon-Transposase Systems for Stable Genetic Modification of Embryonic Stem Cells. In: Methodological Advances in the Culture, Manipulation and Utilization of Embryonic Stem Cells for Basic and Practical Applications, Craig Atwood (Ed.). InTech, ISBN: 978-953-307-197-8; 259-274. (2011)

Popular science papers:

  1. Homolya, L. ABC transzporterek úton útfélen. Biokémia 37:(1) pp. 17-27. (2013)
  2. Fári, K., Homolya, L.. Vezikuláris transzport a kezdetektől a 2013-as Nobel-díjig. Biokémia 37:(4) pp. 27-34. (2013)
  3. Sarkadi B., Apáti Á.: Sejtmagátültetés és újraprogramozás – a 2012-ben orvosi Nobel-díjat kapott kutatók és munkásságuk rövid bemutatása. Magyar Tudomány. 174(6): 643-48. (2013)

Others:

The Research Group possesses a High-Content Screening equipment (Molecular Devices ImageXpress Micro XLS) and two flow cytometers (Beckton Dickinson FACS Canto II és FACSAria III), the latter of which is also capable of cell sorting.