MS Proteomics Research Group

Research projects

1) Neuroproteomics: Description of the synaptic proteome and its plasticity

  • Changes in the synaptic proteome due to sleep deprivation
  • Characterizing of post translational modifications (glycosylation and phosphorylation) present in the synaptic proteome

2) Proteomics and glycoproteomics: Biochemical and clinical applications

  • Characterization of protein mixtures, body fluid and tissue proteomics. Protein sequencing and detection of native protein complexes. Development of novel experimental methodologies for proteomics
  • Glycobiology, analytical characterization of protein glycosylation, and changes in the glycosylation pattern; with focus on site specific N-glycosylation. Changes of glycosylation in pathological processes, development and application of glycosylation based tumor markers for cancer diagnosis
  • Proteomics of extracellular vesicles and exosomes

3) Basic research and (bio)informatics

  • Development and application of bioinformatics for evaluation of experimental results in biochemical and clinical proteomics (GlycoMiner & GlycoPattern)
  • Description fundamentals of tandem mass spectrometry; the role of energetics (MassKinetics)

Equipment

Bruker Maxis II ETD mass spectrometer coupled to Thermo Dionex UltiMate 3000 nanoUPLC
Waters Q-TOF Premier mass spectrometer coupled to nanoAcquity UHPLC
Waters Micromass Quattro-micro mass spectrometer coupled to Acquity UPLC

Cooperation

The MS Proteomics Research group has various collaborations with universities and research groups in Hungary and abroad. We are also doing collaborative and contract research (structure analysis, qualitative and quantitative analysis) for various companies.

Recent Publications

Sódar BW, Kittel Á, Pálóczi K, Vukman KV, Osteikoetxea X, Szabó-Taylor K, Németh A, Sperlágh B, Baranyai T, Giricz Z, Wiener Z, Turiák L, Drahos L, Pállinger É, Vékey K, Ferdinandy P, Falus A, Buzás EI, Low-density lipoprotein mimics blood plasma-derived exosomes and microvesicles during isolation and detection. SCIENTIFIC REPORTS 2016 Apr 18;6:24316. DOI: 10.1038/srep24316.

Bazsó FL, Ozohanics O, Schlosser G, Ludányi K, Vékey K, Drahos L, Quantitative Comparison of Tandem Mass Spectra Obtained on Various Instruments. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY DOI: 10.1007/s13361-016-1408-y. (2016)

Keglevich G, Bagi P, Rapi Z, Bakó P, Drahos L, Szolnoki B, Marosi G, The synthesis of bio-based flame-retarded epoxy-precursors., MACROMOLECULAR SYMPOSIA 352: pp. 46-50. (2015)

Bagi P, Juhász K, Tímári I, Kövér KE, Mester D, Kállay M, Kubinyi M, Szilvási T, Pongrácz P, Kollár L, Karaghiosoff K, Czugler M, Drahos L, Fogassy E, Keglevich G, A study on the optical resolution of 1-isopropyl-3-methyl-3-phospholene 1-oxide and its use the synthesis of borane and platinum complexes, JOURNAL OF ORGANOMETALLIC CHEMISTRY 797: pp. 140-152. (2015)

Rapi Z, Bako P, Drahos L, Keglevich G, Side-Arm Effect of a Methyl alpha-D-Glucopyranoside Based Lariat Ether Catalysts in Asymmetric Syntheses, HETEROATOM CHEMISTRY 26:(1) pp. 63-71. (2015)

Tóth E, Hevér H, Ozohanics O, Telekes A, Vékey K, Drahos L, Simple correction improving long-term reproducibility of HPLC-MS, JOURNAL OF MASS SPECTROMETRY 50:(10) pp. 1130-1135. (2015)

Vékey K, Drahos L, Ludányi K, Tömegspektrometria, In: Sohár Pál (szerk.), A gyógyszerkutatás műszeres módszerei. 424 p., Budapest: Magyar Kémikusok Egyesülete, 2015. pp. 145-170. (ISBN:978-963-9970-61-8) P.)

Völgyi K, Gulyássy P, Háden K, Kis V, Badics K, Kékesi KA, Simor A, Györffy B, Tóth EA, Lubec G, Juhász G, Dobolyi Á, Synaptic mitochondria: a brain mitochondria cluster with a specific proteome, JOURNAL OF PROTEOMICS 120:142-57. (2015)

Marton L, Nagy GN, Ozohanics O, Labas A, Kramos B, Olah J, Vekey K, Vertessy BG, Molecular Mechanism for the Thermo-Sensitive Phenotype of CHO-MT58 Cell Line Harbouring a Mutant CTP:Phosphocholine Cytidylyltransferase, PLOS ONE 10:(6) p. e0129632. (2015)

Kovács Z, Kékesi KA, Dobolyi Á, Lakatos R, Juhász G, Absence Epileptic Activity Changing Effects of Non-Adenosine Nucleoside Inosine, Guanosine and Uridine In Wistar Albino Glaxo Rijswijk Rats. NEUROSCIENCE 300:593-608. (2015)

Völgyi K, Juhász G, Kovács Z, Penke B, Dysfunction of Endoplasmic Reticulum (ER) and Mitochondria (MT) in Alzheimer’s Disease: The Role of the ER-MT Cross-Talk. CURRENT ALZHEIMER RESEARCH 12(7):655-72. (2015)

Kovács Z, Kékesi KA, Juhász G, Dobolyi A, Modulatory effects of inosine, guanosine and uridine on lipopolysaccharide-evoked increase in spike-wave discharge activity in Wistar Albino Glaxo/Rijswijk rats. BRAIN RESEARCH BULLETIN 118:46-57. (2015)

Leader

László Drahos

Members

2018-06-23T08:36:01+00:00 2018. April 11.|Research Groups|
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